Part:BBa_K3790232:Design

T7 gene product 5.7 driven by pBAD/araC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

The following primers were used for constructing this part.

doubleter_f	5-CGATATAAGTTGTAATTCTCaaataataaaaaagccggattaataatctggc
doubleter_r	5-GAAGGCTCTCAAGGGCATtcacactggctcaccttcg
gp5.7_f		5-gaATGCCCTTGAGAGCCTTCTTAGACACATCCTCCCATTCGTTTC
gp5.7_r		5-gagttaaggaggtaaaaaaaATGTCTGACTACCTGAAAGTGCTG
pBAD_f		5-tttttttacctccttaactctagtagctagcc
pBAD_r		5-TGGTGCTACGCCTGAATAAGttatgacaacttgacggctacatcattc
pLysS_f		5-tagccgtcaagttgtcataaCTTATTCAGGCGTAGCACCAG
pLysS_r		5-GAGAATTACAACTTATATCGTATGGGGC
gp5.7_coding1  	5-GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC 	
gp5.7_coding2  	5-CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC	
gp5.7_coding3  	5-ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC                	
gp5.7_template1	5-CCCAAGCGCCACCGTTACGTCCgCTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG	
gp5.7_template2	5-CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG       	
gp5.7_template3	5-TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC       
f_gp5.7_rep2_1722	5-ggtttactttcgtccgcgcgcc          	
f_gp5.7_rep2_1869	5-GGCACTGATGAGGGTGTCAGTGAAGTGC    	
r_gp5.7_rep2_1722	5-CTGACACCCTCATCAGTGCCAACATAGTAAGC	
r_gp5.7_rep2_1869	5-cgcgcggacgaaagtaaacccac